DEFICIENT ACTIVITY OF NUCLEOTIDE BINDING REGULATORY PROTEIN COUPLED WITH PGE2 RECEPTOR IN RENAL MEDULLA OF SPONTANEOUSLY HYPERTENSIVE RATS

被引：15

作者：

YOSHIKAWA, H

FUKUDA, K

BABA, A

NISHIO, H

UEYAMA, T

YOSHIKAWA, A

KUCHII, M

NISHIO, I

MASUYAMA, Y

机构：

[1] The Division of Cardiology, Department of Medicine, Wakayama Medical College, Wakayama

来源：

AMERICAN JOURNAL OF HYPERTENSION | 1990年 / 3卷 / 03期

关键词：

Hypertension; Kidney; Nucleotide binding regulatory protein; Prostaglandin receptor; Spontaneously hypertensive rat;

D O I：

10.1093/ajh/3.3.230

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

Prostaglandin (PG) E2receptor-adenylate cyclase system was studied in the kidney of 12-week-old spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY) to evaluate the role of this system in hypertension. PGE2receptors were determined by a radioligand binding method using [3H]-PGE2. Adenylate cyclase responses to PGE2, sodium fluoride (NaF) and forskolin were also measured. The concentration of PGE2receptor was increased in SHR compared with WKY. PGE2- and NaF-stim-ulated adenylate cyclase activities were significantly lower in SHR than WKY. There was no significant difference in forskolin-stimulated adenylate cyclase activity between SHR and WKY. NaF activates the nucleotide binding regulatory protein (G-protein) and forskolin directly activates the catalytic unit. These results indicate that the activity of G-protein coupled with renal PGE2receptors is deficient in SHR. This defect may contribute to the elevation of blood pressure, through sodium retention. © 1990 by the American Journal of Hypertension, Ltd.

引用

页码：230 / 233

页数：4

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