TARGETING DELETION (HOMOEOLOGOUS CHROMOSOME-PAIRING LOCUS) OR ADDITION LINE SINGLE COPY SEQUENCES FROM CEREAL GENOMES

被引:22
作者
CLARKE, B [1 ]
STANCOMBE, P [1 ]
MONEY, T [1 ]
FOOTE, T [1 ]
MOORE, G [1 ]
机构
[1] JOHN INNES CTR PLANT SCI RES, CAMBRIDGE LAB, COLNEY LANE, NORWICH NR4 7VJ, NORFOLK, ENGLAND
关键词
D O I
10.1093/nar/20.6.1289
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We describe here a protocol for obtaining clones containing sequences present in low copy-number from genomic DNA where moderately and highly repeated sequences predominate. Specific chromosomal regions can be targeted by using deletion or addition line material. We have used this protocol to identify a sequence which has been deleted in both the tetraploid and hexaploid wheat mutants for the homoeologous chromosome pairing locus.
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页码:1289 / 1292
页数:4
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