DETECTION OF MYOSIN GENE-EXPRESSION IN THE DEVELOPING HEART USING PROBES DERIVED BY POLYMERASE CHAIN-REACTION

被引：6

作者：

BARTON, PJR

MOSCOSO, G

THOMPSON, RP

机构：

[1] NATL HEART & LUNG INST,DEPT PAEDIAT,LONDON SW3 6LY,ENGLAND

[2] UNIV LONDON KINGS COLL HOSP,SCH MED,DEPT MORBID ANAT,LONDON SE5 8RX,ENGLAND

来源：

INTERNATIONAL JOURNAL OF CARDIOLOGY | 1991年 / 30卷 / 01期

关键词：

MYOSIN; HYBRIDIZATION; GENE EXPRESSION; CARDIAC DEVELOPMENT;

D O I：

10.1016/0167-5273(91)90133-A

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The polymerase chain reaction provides a rapid method for the molecular cloning of DNA probes suitable for the detection of specific messenger RNA. We have used this approach to prepare probes specific for human cardiac myosin messenger RNA and demonstrate here the use of such probes in the analysis of human cardiac development by hybridization in situ to sections to fetal tissue. This combination of techniques is suitable for the detection of any messenger RNA for which sequence data are available, and offers a powerful new approach to the analysis of cardiac development.

引用

页码：116 / 118

页数：3

共 5 条

[1]

BRAND NJ, IN PRESS GENETIC MAN

[2] A COMPREHENSIVE LIST OF CLONED HUMAN DNA-SEQUENCES [J].

SCHMIDTKE, J ;

COOPER, DN .

NUCLEIC ACIDS RESEARCH, 1990, 18 :2413-2547

[3] MORPHOGENESIS OF HUMAN CARDIAC OUTFLOW [J].

THOMPSON, RP ;

SUMIDA, H ;

ABERCROMBIE, V ;

SATOW, Y ;

FITZHARRIS, TP ;

OKAMOTO, N .

ANATOMICAL RECORD, 1985, 213 (04) :578-586

[4] MOLECULAR-CLONING OF HUMAN CARDIAC TROPONIN-I USING POLYMERASE CHAIN-REACTION [J].

VALLINS, WJ ;

BRAND, NJ ;

DABHADE, N ;

BUTLERBROWNE, G ;

YACOUB, MH ;

BARTON, PJR .

FEBS LETTERS, 1990, 270 (1-2) :57-61

[5]

WILKINSON DG, 1987, DEVELOPMENT, V99, P493

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