SUB-FEMTOMOLE DETERMINATION OF PHENYLTHIOHYDANTOIN-AMINO ACIDS - CAPILLARY ELECTROPHORESIS AND THERMOOPTIC DETECTION

被引：78

作者：

WALDRON, KC ^{[1
]}

DOVICHI, NJ ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON T6G 2G2,ALBERTA,CANADA

来源：

ANALYTICAL CHEMISTRY | 1992年 / 64卷 / 13期

关键词：

D O I：

10.1021/ac00037a017

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Twenty phenylthiohydantoin-amino acids (PTH-AA) are separated by micellar capillary electrophoresis in 13.5 min. A low-energy krypton fluoride laser is used in a thermooptical absorbance detector to produce typical detection limits (3-sigma) of 9 X 10(-7) M PTH-glycine injected in a 0.6-nL volume, corresponding to 0.5 fmol (100 fg) of PTH-glycine injected onto the capillary. Detection limits are roughly 3 orders of magnitude superior to conventional liquid chromatographic determinations. Linear dynamic range for this ultraviolet absorbance detector is greater than 3000 in peak height and at least 20 000 in peak area.

引用

页码：1396 / 1399

页数：4

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