A NOVEL METHOD FOR MEASURING ANTIOXIDANT CAPACITY AND ITS APPLICATION TO MONITORING THE ANTIOXIDANT STATUS IN PREMATURE NEONATES

被引：2554

作者：

MILLER, NJ ^{[1
]}

RICEEVANS, C ^{[1
]}

DAVIES, MJ ^{[1
]}

GOPINATHAN, V ^{[1
]}

MILNER, A ^{[1
]}

机构：

[1] UNIV YORK,DEPT PAEDIAT,YORK YO1 5DD,N YORKSHIRE,ENGLAND

来源：

CLINICAL SCIENCE | 1993年 / 84卷 / 04期

关键词：

ANTIOXIDANT; 2,2'-AZINOBIS-(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID); FERRYL MYOGLOBIN; FREE RADICALS; PREMATURE NEONATES; TROLOX;

D O I：

10.1042/cs0840407

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

1. A new method has been developed for measuring the total antioxidant capacity of body fluids and drug solutions, based on the absorbance of the ABTS.+ radical cation. 2. An automated method for use on a centrifugal analyser, as well as a manual method, is described. 3. The procedure has been applied to physiological antioxidant compounds and radical-scavenging drugs, and an antioxidant ranking was established based on their reactivity relative to a 1.0 mmol/l Trolox standard. 4. The Trolox equivalent antioxidant capacity of plasma from an adult reference population has been measured, and the method optimized and validated. 5. The method has been applied to investigate the total plasma antioxidant capacity of neonates and how this may be compromised in prematurity.

引用

页码：407 / 412

页数：6

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