AFFINITY PURIFICATION OF SYNTHETIC PEPTIDES AND PROTEINS ON POROUS GRAPHITIZED CARBON

被引：28

作者：

BROWN, AR ^{[1
]}

IRVING, SL ^{[1
]}

RAMAGE, R ^{[1
]}

机构：

[1] UNIV EDINBURGH,DEPT CHEM,W MAINS RD,EDINBURGH EH9 3JJ,MIDLOTHIAN,SCOTLAND

来源：

TETRAHEDRON LETTERS | 1993年 / 34卷 / 44期

关键词：

AFFINITY CHROMATOGRAPHY; TBFMOC N-ALPHA-PROTECTION; POROUS GRAPHITIZED CARBON; TETRABENZO[A; C; G; I]FLUORENYL-17-METHANOL; PROTEIN PURIFICATION;

D O I：

10.1016/S0040-4039(00)61617-9

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The affinity of the Tbfmoc group for porous graphitised carbon has been exploited for the purification of synthetic peptides containing up to 85 amino acids. The hydrophobicity of the group has also been used to simplify peptide purification by HPLC.

引用

页码：7129 / 7132

页数：4

共 15 条

[1] POLYAMIDE METHOD OF SOLID-PHASE PEPTIDE AND OLIGONUCLEOTIDE SYNTHESIS
ATHERTON, E
GAIT, MJ
SHEPPARD, RC
WILLIAMS, BJ
[J]. BIOORGANIC CHEMISTRY, 1979, 8 (03) : 351 - 370
[2] BRIAND J-P, 1989, Peptide Research, V2, P381
[3] CHEMOSELECTIVE ONE-STEP PURIFICATION METHOD FOR PEPTIDES SYNTHESIZED BY THE SOLID-PHASE TECHNIQUE
FUNAKOSHI, S
FUKUDA, H
FUJII, N
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) : 6981 - 6985
[4] THE DNA-SEQUENCE OF THE PHAGE LAMBDA-GENOME BETWEEN PL AND THE GENE BET
INEICHEN, K
SHEPHERD, JCW
BICKLE, TA
[J]. NUCLEIC ACIDS RESEARCH, 1981, 9 (18) : 4639 - 4653
[5] KENT SB, 1988, CELL, V54, P363
[6] STRUCTURE AND PERFORMANCE OF POROUS GRAPHITIC CARBON IN LIQUID-CHROMATOGRAPHY
KNOX, JH
KAUR, B
MILLWARD, GR
[J]. JOURNAL OF CHROMATOGRAPHY, 1986, 352 : 3 - 25
[7] PURIFICATION, SEQUENCE, AND CELLULAR-LOCALIZATION OF A NOVEL CHROMOSOMAL PROTEIN THAT BINDS TO METHYLATED DNA
LEWIS, JD
MEEHAN, RR
HENZEL, WJ
MAURERFOGY, I
JEPPESEN, P
KLEIN, F
BIRD, A
[J]. CELL, 1992, 69 (06) : 905 - 914
[8] ON-RESIN BIOTINYLATION OF CHEMICALLY SYNTHESIZED PROTEINS FOR ONE-STEP PURIFICATION
LOBL, TJ
DEIBEL, MR
YEM, AW
[J]. ANALYTICAL BIOCHEMISTRY, 1988, 170 (02) : 502 - 511
[9] LOENEN WAM, 1986, J MOL BIOL, V190, P11, DOI 10.1016/0022-2836(86)90071-9
[10] SOLID-PHASE PEPTIDE-SYNTHESIS OF UBIQUITIN
RAMAGE, R
GREEN, J
OGUNJOBI, OM
[J]. TETRAHEDRON LETTERS, 1989, 30 (16) : 2149 - 2152

← 1 2 →