CLONING AND CHARACTERIZATION OF THE GENE ENCODING CYTOCHROME C(4) FROM PSEUDOMONAS-STUTZERI

被引：15

作者：

CHRISTENSEN, HEM

机构：

[1] Institute of Molecular and Cell Biology, National University of Singapore, Singapore

来源：

GENE | 1994年 / 144卷 / 01期

关键词：

NUCLEOTIDE SEQUENCING; GRAM-NEGATIVE BACTERIA; DI-HEME; RECOMBINANT DNA;

D O I：

10.1016/0378-1119(94)90219-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

By use of a degenerate oligodeoxyribonucleotide probe corresponding to the N-terminal amino acid (aa) sequence and Southern blot analysis, the gene encoding the di-heme cytochrome c(4) (CC4) from Pseudomonas stutzeri has been cloned. The aa sequence deduced from the nucleotide sequence shows a 20-aa signal peptide and a 190-aa mature protein with 79% identity to the Azotobacter vinelandii CC4 sequence, which had earlier been determined by aa sequencing.

引用

页码：139 / 140

页数：2

共 5 条

[1]

AMBLER R P, 1973, Biochemical Society Transactions, V1, P162

[2] THE AMINO-ACID-SEQUENCE OF THE DIHEME CYTOCHROME-C4 FROM THE BACTERIUM AZOTOBACTER-VINELANDII [J].