QUANTITATIVE DETECTION OF HIV-1 DRUG-RESISTANCE MUTATIONS BY AUTOMATED DNA-SEQUENCING

被引：173

作者：

LARDER, BA

KOHLI, A

KELLAM, P

KEMP, SD

KRONICK, M

HENFREY, RD

机构：

[1] APPL BIOSYST 850,FOSTER CITY,CA 94404

[2] APPL BIOSYST LTD,WARRINGTON WA3 7PB,CHESHIRE,ENGLAND

来源：

NATURE | 1993年 / 365卷 / 6447期

关键词：

D O I：

10.1038/365671a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences. Quantitative detection of such mixtures of HIV-1 drug resistance mutations was best achieved using an automated system that uses fluorescent-labelled sequencing primers. This procedure has a wide range of applications in clinical research, including heterozygote analysis. Software that automatically reports mixed-base positions is presented.

引用

页码：671 / 673

页数：3

共 7 条

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