REGULATION OF EICOSANOID RELEASE IN HUMAN UMBILICAL ENDOTHELIAL-CELLS

被引：13

作者：

WEIGEL, G

GRIESMACHER, A

MULLER, MM

机构：

[1] 2nd Dept. of Surgery, Div. of Clinical Biochemistry, University of Vienna, 1090 Vienna

来源：

THROMBOSIS RESEARCH | 1991年 / 62卷 / 06期

关键词：

ENDOTHELIAL CELLS; PROSTAGLANDIN-I2; THROMBOXANE-A2; ADENINE NUCLEOTIDES; CAMP; CGMP;

D O I：

10.1016/0049-3848(91)90372-4

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The concentration dependent influence of adenine nucleotides, thrombin and ionophore A 23187 on eicosanoid-production of human endothelial cells was investigated. The prostaglandin I2 (PGI2)-and thromboxane A2 (TXA2)-release were highest at concentrations of 1 mmol/l and 4 mmol/l adenine nucleotides, respectively. TXA2-release decreased at higher concentrations. Ionophore-induced eicosanoid-formation reached its maximum at 15-mu-mol/l for both PGI2 and TXA2. Thrombin stimulation resulted in a bell shaped concentration dependency, whereby most TXA2 was released at a concentration of 1 U/ml and most PGI2 at 5 U/ml. Intracellular cAMP showed significant (p < 0.01) increases stimulating cells with ADP- and ATP-concentrations higher than 500-mu-mol/l, whereas ionophore and thrombin did hardly affect cAMP levels.

引用

收藏

页码：685 / 695

页数：11

相关论文

共 21 条

[1] ROLE OF CA2+ AND CYCLIC-AMP IN THE REGULATION OF THE PRODUCTION OF PROSTACYCLIN BY THE VASCULAR ENDOTHELIUM [J].

BROTHERTON, AFA ;

HOAK, JC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (02) :495-499

[2] ADENOSINE-TRIPHOSPHATE STIMULATES INOSITOL PHOSPHOLIPID-METABOLISM AND PROSTACYCLIN FORMATION IN ADRENAL-MEDULLARY ENDOTHELIAL-CELLS BY MEANS OF P2-PURINERGIC RECEPTORS [J].

FORSBERG, EJ ;

FEUERSTEIN, G ;

SHOHAMI, E ;

POLLARD, HB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (16) :5630-5634

[3]

Gerlach E, 1987, TOPICS PERSPECTIVES, P454

[4] EXTRACELLULAR ATP - EFFECTS, SOURCES AND FATE [J].

GORDON, JL .

BIOCHEMICAL JOURNAL, 1986, 233 (02) :309-319

[5] THROMBOXANE-A2 GENERATION BY HUMAN UMBILICAL ENDOTHELIAL-CELLS [J].

GRIESMACHER, A ;

WEIGEL, G ;

SCHREINER, W ;

MULLER, MM .

THROMBOSIS RESEARCH, 1989, 56 (05) :611-623

[6] FINE-STRUCTURE OF VASCULAR ENDOTHELIUM IN CULTURE [J].

HAUDENSCHILD, CC ;

COTRAN, RS ;

GIMBRONE, MA ;

FOLKMAN, J .

JOURNAL OF ULTRASTRUCTURE RESEARCH, 1975, 50 (01) :22-32

[7] PRODUCTS, KINETICS, AND SUBSTRATE-SPECIFICITY OF HOMOGENEOUS THROMBOXANE SYNTHASE FROM HUMAN-PLATELETS - DEVELOPMENT OF A NOVEL ENZYME ASSAY [J].

HECKER, M ;

HAURAND, M ;

ULLRICH, V ;

DICZFALUSY, U ;

HAMMARSTROM, S .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1987, 254 (01) :124-135

[8] REGULATION OF ENDOTHELIAL-CELL CYCLIC NUCLEOTIDE-METABOLISM BY PROSTACYCLIN [J].

HOPKINS, NK ;

GORMAN, RR .

JOURNAL OF CLINICAL INVESTIGATION, 1981, 67 (02) :540-546

[9] BOVINE ENDOTHELIAL-CELLS IN CULTURE PRODUCE THROMBOXANE AS WELL AS PROSTACYCLIN [J].

INGERMANWOJENSKI, C ;

SILVER, MJ ;

SMITH, JB ;

MACARAK, E .

JOURNAL OF CLINICAL INVESTIGATION, 1981, 67 (05) :1292-1296

[10] CULTURE OF HUMAN ENDOTHELIAL CELLS DERIVED FROM UMBILICAL VEINS - IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGICAL CRITERIA [J].

JAFFE, EA ;

NACHMAN, RL ;

BECKER, CG ;

MINICK, CR .

JOURNAL OF CLINICAL INVESTIGATION, 1973, 52 (11) :2745-2756