A CLUSTER OF STRONG TOPOISOMERASE-II CLEAVAGE SITES IS LOCATED NEAR AN INTEGRATED HUMAN-IMMUNODEFICIENCY-VIRUS

被引：35

作者：

HOWARD, MT ^{[1
]}

GRIFFITH, JD ^{[1
]}

机构：

[1] UNIV N CAROLINA,LINEBERGER COMPREHENS CANC CTR,CHAPEL HILL,NC 27599

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 232卷 / 04期

关键词：

TOPOISOMERASE-II; ELECTRON MICROSCOPY; DNA CLEAVAGE; HIV; INVERTED REPEATS;

D O I：

10.1006/jmbi.1993.1460

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Human Immunodeficiency Virus (HIV) integrates into host cellular DNA as a double strand DNA molecule. Here a previously studied HIV isolate was examined for binding and cleavage by topoisomerase II in vitro within the 5’ LTR region and human flanking DNA. A cluster of strong binding and cleavage sites in the human sequences was located approximately 850 bp upstream from the integration site. This region maps to a locus consisting of a complex repeating element, and alternating purine/pyrimidine sequences. Topoisomerase II binding and cleavage sites were also located within the HIV 5’ LTR, in particular a site overlying the DNA sequence coding for TAR, another inverted repeat element in the DNA. © 1993 Academic Press Limited.

引用

页码：1060 / 1068

页数：9

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