CHEMILUMINESCENT DETERMINATION OF ALKALINE-PHOSPHATASE ACTIVITY IN SERUM

A highly sensitive and rapid chemiluminescent assay for the determination of the activity of alkaline phosphatase (ALP) in serum was developed by monitoring the chemiluminescent signal obtained from an ALP triggerable dioxetane, adamantyl-1,2-dioxetane phenyl phosphate (PPD) used as substrate for the enzyme. The response was linear from 0.1 to 500 U 1-1 at 25-degrees-C for standard (buffered) ALP solutions and from 10 to 1000 U 1-1 at 25-degrees-C for standard ALP added to serum. The intra- and inter-assay relative standard deviations were less than 6 % and recoveries ranged from 91 to 106 %. Results agreed well with those obtained with a spectrophotometric method for the determination of total ALP activity in serum and for liver- and bone-type ALP isoenzymes activity.