IN-VIVO OBSERVATION WITH MAGNETIC-RESONANCE-IMAGING OF MIDDLE-EAR EFFUSION IN RESPONSE TO EXPERIMENTAL UNDERPRESSURES

In this study, magnetic resonance imaging (MRI) was used to define, in vivo the effect of acute middle ear (ME) underpressures on vascular permeability and the development of effusion, The MEs of four cynomolgus monkeys were unilaterally inflated with oxygen and carbon dioxide on different occasions and followed for a period of approximately 4 hours by tympanometry and MRI scanning. Carbon dioxide inflations caused the rapid development of ME underpressures of less than -600 mmH2O by 10 minutes. The MRI scans showed a progressive brightening of the ME and ah associated air cells, indicative of the accumulation of effusion in three of the four experiments. An MRI contrast agent was administered to the vascular compartment during the course of the experiment and was rapidly transferred to the ME space, indicating vascular permeability to the agent. The contralateral, control side did not develop significant underpressures, effusion, or increased vascular permeability. Inflation with oxygen caused lesser underpressures and no accompanying changes in the MRI scans. These data support the hydrops ex vacuo theory and confirm the usefulness of MRI for in vivo documentation of the development of ME effusions and changes in vascular permeability of the mucosa in the experimental setting.