ELECTROPHORETIC BEHAVIOR OF MICELLAR AND MONOMERIC SODIUM DODECYL-SULFATE IN POLYACRYLAMIDE-GEL ELECTROPHORESIS WITH REFERENCE TO THOSE OF SDS-PROTEIN COMPLEXES

被引：15

作者：

KUBO, K ^{[1
]}

ISEMURA, T ^{[1
]}

TAKAGI, T ^{[1
]}

机构：

[1] OSAKA UNIV, INST PROT RES, SUITA, OSAKA 565, JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 92卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90652-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrophoretic behavior of sodium dodecyl sulfate (SDS) in polyacrylamide gels has been examined at various gel concentrations. Micellar SDS is subject to significant molecular sieving from the gels while monomeric SDS is virtually free from the effect. The two forms are in a rapid equilibrium with each other. The gel concentration, therefore, has a signifieant effect on the electrophoresis of SDS added in SDS-polyacrylamide gel electrophoresis. The simplified procedure of Stoklosa and Latz (1974, Biochem. Biophys. Res. Commun. 58, 74-79), in which SDS is added only in sample solutions, has been criticized based on the results obtained. © 1979.

引用

页码：243 / 247

页数：5

共 8 条

[1] ELECTROPHORETIC PROPERTIES OF SODIUM DODECYL-SULFATE AND RELATED CHANGES IN ITS CONCENTRATION IN SDS-POLYACRYLAMIDE GEL-ELECTROPHORESIS [J].