PROPERTIES OF THE ESCHERICHIA-COLI RUVA AND RUVB PROTEINS INVOLVED IN DNA-REPAIR, RECOMBINATION AND MUTAGENESIS

被引：15

作者：

SHINAGAWA, H

SHIBA, T

IWASAKI, H

MAKINO, K

TAKAHAGI, M

NAKATA, A

机构：

[1] Department of Experimental Chemotheraphy, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka

来源：

BIOCHIMIE | 1991年 / 73卷 / 04期

关键词：

DNA REPAIR; MUTAGENESIS; RECOMBINATION; RUVA; RUVB; SOS REGULATION;

D O I：

10.1016/0300-9084(91)90120-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ruvA and ruvB genes constitute an operon, which is regulated by the SOS system and involved in DNA repair, recombination and mutagenesis. RuvA protein binds to both single-stranded and double-stranded DNA. RuvB protein has weak ATPase activity. RuvA bound to DNA greatly enhances ATPase activity of RuvB. UV-irradiation to supercoiled DNA further enhances the stimulatory effect of RuvA on the RuvB ATPase activity. In the presence of ATP the RuvA-RuvB complex has an activity that renatures cruciform structures formed by heating and gradually cooling supercoiled DNA with an inverted repeat. These findings suggest that the RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication.

引用

页码：505 / 507

页数：3

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