ENZYMATIC DEGRADATION AND IMMUNOGENIC PROPERTIES OF DERIVATIZED DEXTRANS

被引:41
作者
CREPON, B
JOZEFONVICZ, J
CHYTRY, V
RIHOVA, B
KOPECEK, J
机构
[1] UNIV PARIS 13, RECH MACROMOLEC LAB, CNRS, URA 502, AVE JB CLEMENT, F-93430 VILLETANEUSE, FRANCE
[2] CZECHOSLOVAK ACAD SCI, INST MACROMOLEC CHEM, CS-11142 PRAGUE 1, CZECHOSLOVAKIA
[3] CZECHOSLOVAK ACAD SCI, INST MICROBIOL, CS-11142 PRAGUE 1, CZECHOSLOVAKIA
[4] UNIV UTAH, DEPT PHARMACEUT, SALT LAKE CITY, UT 84112 USA
[5] UNIV UTAH, DEPT BIOENGN, SALT LAKE CITY, UT 84112 USA
关键词
DERIVATED DEXTRAN; ENZYMATIC DEGRADATION; IMMUNOGENICITY;
D O I
10.1016/0142-9612(91)90049-G
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Dextran T40 was modified with carboxylic, benzylamide and benzylamine-sulphonated groups (samples 1-10). The polymers were incubated with dextranase and the decrease of molecular weight was determined by high performance size exclusion chromatography. It was shown that the higher the substitution of dextrans, the lower their degradability. Modification of dextran with benzylamine and benzylamine-sulphonated groups appeared to hinder the formation of the enzyme-substrate complex more than the same quantity of carboxylic groups. The immunogenicity of one of the modified dextrans, containing 54% of carboxylic groups and 19.5% of benzylamine-sulphonated groups, was determined after subcutaneous and intravenous administration into Balb/c mice. The antibody titre was very low even if administered in complete Freund's adjuvant and did not depend on the injected dose. On the average, the titres of antibodies were lower by five orders of magnitude compared to bovine gamma globulin.
引用
收藏
页码:550 / 554
页数:5
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