A BIRCH GENE FAMILY ENCODING POLLEN ALLERGENS AND PATHOGENESIS-RELATED PROTEINS

被引：55

作者：

SWOBODA, I

SCHEINER, O

KRAFT, D

BREITENBACH, M

HEBERLEBORS, E

VICENTE, O

机构：

[1] UNIV VIENNA,VIENNA BIOCTR,INST MICROBIOL & GENET,A-1030 VIENNA,AUSTRIA

[2] UNIV VIENNA,GEN HOSP,INST GEN & EXPTL PATHOL,A-1090 VIENNA,AUSTRIA

[3] SALZBURG UNIV,GENET INST,A-5020 SALZBURG,AUSTRIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1994年 / 1219卷 / 02期

基金：

奥地利科学基金会;

关键词：

ALLERGY; BET V I; MICROBIAL PATHOGEN; POLLEN; PATHOGENESIS-RELATED PROTEIN; (BETULA);

D O I：

10.1016/0167-4781(94)90072-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bet upsilon I, the major pollen allergen of birch (Betula verrucosa), shows high sequence homology to a family of pathogenesis-related (PR) proteins that have recently been identified in several other plant species. We have used a pollen Bet upsilon I cDNA clone and anti-Bet upsilon I antibodies as probes to study the expression of Bet upsilon I genes in birch cell suspension cultures under different experimental conditions. Induction of Bet upsilon I-related proteins was detected in immunoblots of cell extracts upon co-cultivation with microbial pathogens. Northern analysis revealed the rapid induction of Bet upsilon I transcripts in the presence of bacteria and fungi, but not by stress treatments (heat shock, metal ions) or by chemical elicitors. RNase protection experiments showed that the pathogen-inducible RNAs did not correspond to the pollen cDNA clone but most likely to the products of transcription of other members of the Bet upsilon I gene family, sharing high sequence homology with the pollen-specific gene within the 5'-half of the coding region. We conclude that the Bet upsilon I gene family of pollen allergens includes a subset of defense-related genes that are transcriptionally activated in the presence of microbial pathogens.

引用

页码：457 / 464

页数：8

共 29 条

[1]

[Anonymous], 1989, GENETIC ENG PRINCIPL

[2]

BOWLES DJ, 1990, ANNU REV BIOCHEM, V59, P873, DOI 10.1146/annurev.bi.59.070190.004301

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] THE GENE CODING FOR THE MAJOR BIRCH POLLEN ALLERGEN BETVL, IS HIGHLY HOMOLOGOUS TO A PEA DISEASE RESISTANCE RESPONSE GENE [J].