HRP(-) MUTANTS OF PSEUDOMONAS-SOLANACEARUM AS POTENTIAL BIOCONTROL AGENTS OF TOMATO BACTERIAL WILT

被引:57
作者
FREY, P
PRIOR, P
MARIE, C
KOTOUJANSKY, A
TRIGALETDEMERY, D
TRIGALET, A
机构
[1] INRA, CNRS, BIOL MOLEC RELAT PLANTES MICROORGANISMES LAB, F-31326 CASTANET TOLOSAN, FRANCE
[2] INRA, PATHOL VEGETALE LAB, F-75231 PARIS 05, FRANCE
[3] INRA, STN PATHOL VEGETALE PHYTOECOL & MALHERBOL, F-97185 POINTE A PITRE, Guadeloupe, FRANCE
关键词
D O I
10.1128/AEM.60.9.3175-3181.1994
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
There have been many attempts to control bacterial with with antagonistic bacteria or spontaneous nonpathogenic mutants of Pseudomonas solanacearum that lack the ability to colonize the host, but they have met with limited success. Since a large gene cluster (hrp) is involved in the pathogenicity of P. solanacearum, we developed a biological control strategy using genetically engineered Hrp(-) mutants of P. solanacearum. Three pathogenic strains collected in Guadeloupe (French West Indies) were rendered nonpathogenic by insertion of an Omega-Km interposon within the hrp gene cluster of each strain. The resulting Hrp(-) mutants were tested for their ability to control bacterial wilt in challenge inoculation experiments conducted either under growth chamber conditions or under greenhouse conditions in Guadeloupe. Compared with the colonization by a pathogenic strain which spread throughout the tomato plant, colonization by the mutants was restricted to the roots and the lower part of the stems. The mutants did not reach the fruit. Moreover, the presence of the mutants did not affect fruit production. When the plants were challenge inoculated with a pathogenic strain, the presence of Hrp(-) mutants within the plants was correlated with a reduction in disease severity, although pathogenic bacteria colonized the stem tissue at a higher density than the nonpathogenic bacteria. Challenge inoculation experiments conducted under growth chamber conditions led, in some cases, to exclusion of the pathogenic strain from the aerial part of the plant, resulting in high protection rates. Furthermore, there was evidence that one of the pathogenic strains used for the challenge inoculations produced a bacteriocin that inhibited the in vitro growth of the nonpathogenic mutants.
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页码:3175 / 3181
页数:7
相关论文
共 35 条
[1]   BIOLOGICAL-CONTROL OF BACTERIAL WILT CAUSED BY PSEUDOMONAS-SOLANACEARUM IN INDIA WITH ANTAGONISTIC BACTERIA [J].
ANURATHA, CS ;
GNANAMANICKAM, SS .
PLANT AND SOIL, 1990, 124 (01) :109-116
[2]   AN ANTIMICROBIAL SUBSTANCE PRODUCED BY PSEUDOMONAS-CEPACIA B5 AGAINST THE BACTERIAL WILT DISEASE PATHOGEN, PSEUDOMONAS-SOLANACEARUM [J].
AOKI, M ;
UEHARA, K ;
KOSEKI, K ;
TSUJI, K ;
IIJIMA, M ;
ONO, K ;
SAMEJIMA, T .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1991, 55 (03) :715-722
[3]  
Ausubel FM., 1990, CURRENT PROTOCOLS MO
[4]  
Boucher C. A., 1988, Molecular Plant-Microbe Interactions, V1, P282, DOI 10.1094/MPMI-1-282
[5]   PSEUDOMONAS-SOLANACEARUM GENES-CONTROLLING BOTH PATHOGENICITY ON TOMATO AND HYPERSENSITIVITY ON TOBACCO ARE CLUSTERED [J].
BOUCHER, CA ;
VANGIJSEGEM, F ;
BARBERIS, PA ;
ARLAT, M ;
ZISCHEK, C .
JOURNAL OF BACTERIOLOGY, 1987, 169 (12) :5626-5632
[6]  
BOUCHER CA, 1985, J GEN MICROBIOL, V131, P2449
[7]   EFFECTS OF AVIRULENT BACTERIOCIN-PRODUCING STRAINS OF PSEUDOMONAS-SOLANACEARUM ON THE CONTROL OF BACTERIAL WILT OF TOBACCO [J].
CHEN, WY ;
ECHANDI, E .
PLANT PATHOLOGY, 1984, 33 (02) :245-253
[8]   MULTIPLICATION OF PSEUDOMONAS-SOLANACEARUM IN RESISTANT POTATO PLANTS AND THE ESTABLISHMENT OF LATENT INFECTIONS [J].
CIAMPI, L ;
SEQUEIRA, L .
AMERICAN POTATO JOURNAL, 1980, 57 (07) :319-329
[9]   BIOLOGICAL-CONTROL OF BACTERIAL WILT OF POTATOES CAUSED BY PSEUDOMONAS-SOLANACEARUM [J].
CIAMPIPANNO, L ;
FERNANDEZ, C ;
BUSTAMANTE, P ;
ANDRADE, N ;
OJEDA, S ;
CONTRERAS, A .
AMERICAN POTATO JOURNAL, 1989, 66 (05) :315-332
[10]   INTERACTION OF PSEUDOMONAS-SOLANACEARUM LIPOPOLYSACCHARIDE AND EXTRACELLULAR POLYSACCHARIDE WITH AGGLUTININ FROM POTATO-TUBERS [J].
DUVICK, JP ;
SEQUEIRA, L .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 48 (01) :192-198