EXPRESSION AND SOLUBILIZATION OF A RECOMBINANT HUMAN NEUROKININ-1 RECEPTOR IN INSECT CELLS

被引：17

作者：

MAZINA, KE ^{[1
]}

STRADER, CD ^{[1
]}

FONG, TM ^{[1
]}

机构：

[1] MERCK SHARP & DOHME LTD,RES LABS,DEPT MOLEC PHARMACOL & BIOCHEM,RAHWAY,NJ 07065

来源：

JOURNAL OF RECEPTOR RESEARCH | 1994年 / 14卷 / 01期

关键词：

D O I：

10.3109/10799899409066997

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The human neurokinin-l receptor has been expressed in insect cells using a recombinant baculovirus. The expression level is about 10 times higher than that obtained in mammalian cells. The recombinant receptor was solubilized with CHAPS, and a PEG precipitation procedure was shown to be effective in regaining high affinity substance P binding. This system should allow large scale purification of the human neurokinin-1 receptor.

引用

页码：63 / 73

页数：11

共 20 条

[1] MODULATION OF NEUROGENIC INFLAMMATION - NOVEL APPROACHES TO INFLAMMATORY DISEASE [J].

BARNES, PJ ;

BELVISI, MG ;

ROGERS, DF .

TRENDS IN PHARMACOLOGICAL SCIENCES, 1990, 11 (05) :185-189

[2]

CASCIERI MA, 1992, MOL PHARMACOL, V41, P1096

[3]

CASCIERI MA, 1992, MOL PHARMACOL, V42, P458

[4] SUBSTANCE P-MEDIATED SLOW EXCITATORY POSTSYNAPTIC POTENTIAL ELICITED IN DORSAL HORN NEURONS INVIVO BY NOXIOUS-STIMULATION [J].

DE KONINCK, Y ;

HENRY, JL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (24) :11344-11348

[5] CALCULATING RECEPTOR NUMBER FROM BINDING EXPERIMENTS USING SAME COMPOUND AS RADIOLIGAND AND COMPETITOR [J].

DEBLASI, A ;

OREILLY, K ;

MOTULSKY, HJ .

TRENDS IN PHARMACOLOGICAL SCIENCES, 1989, 10 (06) :227-229

[6]

FONG TM, 1992, J BIOL CHEM, V267, P25664

[7] THE EXTRACELLULAR DOMAIN OF THE NEUROKININ-1 RECEPTOR IS REQUIRED FOR HIGH-AFFINITY BINDING OF PEPTIDES [J].

FONG, TM ;

YU, H ;

HUANG, RRC ;

STRADER, CD .

BIOCHEMISTRY, 1992, 31 (47) :11806-11811

[8]

FONG TM, 1992, MOL PHARMACOL, V41, P24

[9]

FONG TM, 1992, J BIOL CHEM, V267, P25688

[10] DIFFERENT BINDING EPITOPES ON THE NK1 RECEPTOR FOR SUBSTANCE-P AND A NONPEPTIDE ANTAGONIST [J].

GETHER, U ;

JOHANSEN, TE ;

SNIDER, RM ;

LOWE, JA ;

NAKANISHI, S ;

SCHWARTZ, TW .

NATURE, 1993, 362 (6418) :345-348

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