DETERMINATION OF A CYSTEINE PROTEASE INHIBITOR AND ITS ETHYL-ESTER IN MOUSE SERUM AND MUSCLE BY LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：6

作者：

KAI, M ^{[1
]}

TAKANO, C ^{[1
]}

NOHTA, H ^{[1
]}

OHKURA, Y ^{[1
]}

机构：

[1] KYUSHU UNIV 62,FAC PHARMACEUT SCI,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 621卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)80094-K

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A liquid chromatographic-mass spectrometric method is described for the determination of a cysteine protease inhibitor (E64C) and its ethyl ester in mouse serum and muscle samples. The compounds in the sample, after deproteinization and solid-phase extraction, were separated by isocratic reversed-phase high-performance liquid chromatography and detected by on-line mass spectrometry. The use of an aqueous mobile phase containing methanol and 30 mM ammonium trichloroacetate provided abundant protonated molecular ions of the compounds in the atmospheric pressure chemical ionization interface of the detection system. The method permitted the quantitative determination of the inhibitors without internal standards in the biological matrices. The detections limits for the compounds, in the selected-ion monitoring mode, were 10-15 pmol on-column, at a signal-to-noise ratio of 5.

引用

页码：181 / 187

页数：7

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