QUANTIFICATION OF FREE ALPHA-2-MACROGLOBULIN AND ALPHA-2-MACROGLOBULIN-PROTEASE COMPLEXES BY A NOVEL ELISA SYSTEM BASED ON STREPTOCOCCAL ALPHA-2-MACROGLOBULIN RECEPTORS

被引：8

作者：

JUSTUS, CWE

MULLER, HP

SIMON, MM

KRAMER, MD

机构：

[1] UNIV HEIDELBERG,HAUT KLIN,FORSCHUNGSGRP,NEUENHEIMER FELD 324,W-6900 HEIDELBERG,GERMANY

[2] MAX PLANCK GESELL,KLIN FORSCHUNGSGRP BLUTGERINNUNG & THROMBOSE,W-6300 GIESSEN,GERMANY

[3] SWEDISH UNIV AGR SCI,DEPT MICROBIOL,S-75007 UPPSALA,SWEDEN

[4] MAX PLANCK INST IMMUNBIOL,W-7800 FREIBURG,GERMANY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 126卷 / 01期

关键词：

ELISA; α[!sub]2[!/sub]-Macroglobulin; α[!sub]2[!/sub]-Macroglobulin protease complex;

D O I：

10.1016/0022-1759(90)90017-P

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

An ELISA test system has been developed for the quantification of the two distinct forms of the proteinase inhibitor α2-macroglobulin (α2M): (i) free α2M (functionally active), which is the electrophoretically slow form (α2MS), and (ii) the α2M-proteinase complex (functionally inactive), which is the elctrophoretically fast form of α2M (α2MF). Discrimination between the two types of α2M was achieved using extracts of the two independent streptococcal strains, M1 and Sc1, which express receptors for α2MS and α2MF, respectively, in combination with a monoclonal antibody specific for α2M. The assay system described is easy and reliable and permits quantitation of α2MS and α2MF in complex biological samples such as plasma and cutaneous suction blister fluid. © 1990.

引用

页码：103 / 108

页数：6

共 14 条

[1]

ELECTROPHORETICALLY SLOW AND FAST FORMS OF THE ALPHA-2-MACROGLOBULIN MOLECULE [J].