ENZYMATIC-ACTIVITY AND IMMUNOREACTIVITY OF EXTRACELLULAR PHOSPHOLIPASE-A2 IN INFLAMMATORY SYNOVIAL-FLUIDS

被引：42

作者：

PRUZANSKI, W ^{[1
]}

SCOTT, K ^{[1
]}

SMITH, G ^{[1
]}

RAJKOVIC, I ^{[1
]}

STEFANSKI, E ^{[1
]}

VADAS, P ^{[1
]}

机构：

[1] GARVAN INST MED RES,DARLINGHURST,AUSTRALIA

来源：

INFLAMMATION | 1992年 / 16卷 / 05期

关键词：

D O I：

10.1007/BF00918971

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Synovial fluid PLA2 concentration was measured by an ELISA technique using monoclonal antibodies raised against human recombinant "synovial-type" group II phospholipase A2. This ELISA was specific for synovial-type PLA2 and did not detect pancreatic (group I) PLA2. In all synovial fluids examined, including rheumatoid, osteoarthritic, psoriatic, and gouty fluids, synovial fluid PLA2 enzyme activity significantly correlated with PLA2 immunoreactivity (P < 0.001). Within the limits of the ELISA technique, there was no evidence for the presence of specific or nonspecific modulation of PLA2 activity by either putative PLA2 activating or inhibitory proteins.

引用

页码：451 / 457

页数：7

共 26 条

[1]

BOMALASKI JS, 1991, J IMMUNOL, V146, P3904

[2]

BOMALASKI JS, 1990, J LAB CLIN MED, V116, P814

[3] HUMAN RECOMBINANT LIPOCORTIN-1 HAS ACUTE LOCAL ANTI-INFLAMMATORY PROPERTIES IN THE RAT PAW EDEMA TEST [J].