MICRO-INJECTION OF RICIN ENTRAPPED IN UNILAMELLAR LIPOSOMES INTO A RICIN-RESISTANT MUTANT OF BABY HAMSTER-KIDNEY CELLS

被引：10

作者：

GARDAS, A ^{[1
]}

MACPHERSON, I ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND LABS,LONDON WC2A 3PX,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 584卷 / 03期

关键词：

(BHK cell); Liposome; Ricin resistance;

D O I：

10.1016/0304-4165(79)90126-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescein-labelled Ricin was entrapped in unilamellar liposomes; 14 μg of protein was entrapped by 1 mg of lipids. Liposomes added to cells in culture in low serum medium can deliver entrapped Ricin to a Ricin-resistant mutant of baby hamster kidney(BHK)cells. Ricin entrapped in unilamellar liposomes inhibits protein biosynthesis at a concentration of 1.75 μg/ml in Ricin-resistant cells. Ricin dissolved in medium at 50 μg/ml does not affect protein synthesis in these cells. © 1979.

引用

页码：538 / 541

页数：4

共 21 条

[1] SINGLE BILAYER LIPOSOMES PREPARED WITHOUT SONICATION [J].