SPECIFIC BINDING-SITES FOR PROSTAGLANDIN-D2 ON HUMAN-PLATELETS

被引：52

作者：

SIEGL, AM ^{[1
]}

SMITH, JB ^{[1
]}

SILVER, MJ ^{[1
]}

机构：

[1] THOMAS JEFFERSON UNIV,DEPT PHARMACOL,PHILADELPHIA,PA 19107

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 01期

关键词：

D O I：

10.1016/0006-291X(79)91623-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

3H-PGD2 was biosynthesized from 3H-arachidonate and used to study the binding of PGD2 to intact human platelets. The binding of 3H-PGD2 to platelets was rapid, being essentially complete within two min. Bound 3H-PGD2 PGD2. Scatchard analysis of concentration-dependent binding indicated a single class of binding sites with a dissociation constant (KD) of 4.12 × 10-7M and a capacity of 760 sites per platelet. The relative ability of PGD2, PGE2, PGE1 and PGI2 to displace 3H-PGD2 bound to these sites was 100:2:2<1. We conclude therefore, that these PGD2 binding sites are specific for PGD2 and independent of those previously demonstrated to recognize 3H-PGI2 and 3H-PGE1. © 1979.

引用

页码：291 / 296

页数：6

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