INVESTIGATION OF THE SYSTEM COBALT(II) BOVINE CARBONIC-ANHYDRASE B-TRICHLOROACETALDEHYDE

被引:9
作者
BERTINI, I [1 ]
BORGHI, E [1 ]
CANTI, G [1 ]
LUCHINAT, C [1 ]
机构
[1] CNR,STUDIO COMPOSTI COORDINAZIONI LAB,FLORENCE,ITALY
关键词
D O I
10.1016/S0162-0134(00)80053-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The interactions between hydrated trichloroacetaldehyde and cobalt(II)bovine carbonic anhydrase B have been investigated as a function of pH by means of electronic spectroscopy of FT nmr spectroscopy. The hydrated aldehyde is bound to the metal ion and its apparent affinity constant is pH dependent with a bell-shaped profile. The kinetic parameters of the dissociation process have also been determined. © 1979.
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页码:49 / 56
页数:8
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