USE OF BARIUM SALTS OF PROTECTED DEOXYRIBONUCLEOSIDE-3' P-CHLOROPHENYL PHOSPHATES FOR CONSTRUCTION OF OLIGONUCLEOTIDES BY THE PHOSPHOTRIESTER METHOD - HIGH-YIELD SYNTHESIS OF DINUCLEOTIDE BLOCKS

A new experimental approach to the synthesis of polydeoxyribonucleotides via the phosphotriester method involves construction of oligonucleotide blocks by direct use of the easily prepared barium salts of O5′.N-protected deoxyribonucleoside-3′ p-chlorophenyl phosphates aa the key monomers in condensation reactions. The procedure has been demonstrated by the rapid synthesis in high yield and purity of all sixteen fully protected dinucleotides [(MeO)2Tr]dN′-dN′°.-(CNEt), (where dN′ · dT, dbzC, dbzA, or dibG; ° · diesterifled p-chlorophenyl phosphate). This set of molecules constitutes a syllabary" for the preparation of defined sequence oligonucleotides. © 1979 Information Retrieval Limited."