INHIBITION OF PLANT PHYTASES BY PHLOROGLUCINOL

被引：4

作者：

GOEL, M ^{[1
]}

SHARMA, CB ^{[1
]}

机构：

[1] MEERUT UNIV,DAV COLL,POST GRAD DEPT CHEM,BIOCHEM LABS,MUZAFFARNAGAR 251002,INDIA

来源：

PHYTOCHEMISTRY | 1979年 / 18卷 / 06期

关键词：

Cucurbita maxima; Cucurbitaceae; inhibitor; phloroglucinol; phytase; phytic acid; polyphenol; pumpkin seeds;

D O I：

10.1016/S0031-9422(00)91453-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant phytases (myoinositol hexaphosphate phosphohydrolase, EC 3.1.3.8) were inhibited by phloroglucinol (1,3,5-benzenetriol) in vitro. The inhibition of the Cucurbita maxima phytase was found to be non-competitive and pH dependent with an apparent inhibition constant (Ki) value of 2.3 × 10-1 M at optimum pH (4.8) and temperature (50°). The apparent number of inhibitor molecules (n) bound per enzyme molecule was found to be 2.2 suggesting that the positive cooperativity phenomenon may be present. © 1979.

引用

页码：939 / 942

页数：4

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