MODIFIED GIEMSA STAIN FOR POLYMETHYL METHACRYLATE EMBEDDED UNDECALCIFIED BONE AND BONE-MARROW SPECIMENS

被引：9

作者：

DELEON, ER

ARMSTRONG, R

机构：

[1] Celtrix Laboratories, Collagen Corporation, Palo Alto, CA, 94303

来源：

JOURNAL OF HISTOTECHNOLOGY | 1991年 / 14卷 / 02期

关键词：

BONE MARROW; GIEMSA; PMMA PLASTIC SECTIONS; UNDECALCIFIED BONE;

D O I：

10.1179/his.1991.14.2.85

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A modified Giemsa azure-eosin method for staining polymethyl methacrylate (PMMA) plastic embedded bone sections is described. The method is similar to those used to stain glycol methacrylate sections but differs in timing and addition of air drying steps. The stain yields effective differentiation of marrow cells, growth plate, calcified cartilage, osteoid, bone matrix and bone cells in undecalcified sections of bone. The stain does not interfere with the resolution of fluorochrome labeled specimens. Hence, the same slide can be used to evaluate static and dynamic parameters of bone metabolism.

引用

页码：85 / 88

页数：4

共 8 条

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