A COSMID CLONE FOR THE 5HT1A RECEPTOR (HTR1A) REVEALS A TAQI RFLP THAT SHOWS TIGHT LINKAGE TO DNA LOCI D5S6, D5S39, AND D5S76

被引：11

作者：

MELMER, G

SHERRINGTON, R

MANKOO, B

KALSI, G

CURTIS, D

GURLING, HMD

机构：

[1] Molecular Psychiatry Laboratory, Academic Department of Psychiatry, University College, London WIP TPN, Riding House Street

来源：

GENOMICS | 1991年 / 11卷 / 03期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0888-7543(91)90088-V

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A human neuroreceptor clone (G21), which was isolated by cross-hybridization with the human clone for the β2-adrenergic receptor, has recently been shown to encode the gene for the 5HT1A receptor (HTR1A) subtype. In situ hybridization to human metaphase chromosomes mapped the G21 sequence to chromosome 5 at bands 5q11.2-q13. The clone G21 recognizes a SacI RFLP with low heterozygosity (0.13). To increase the informativeness of the HTR1A locus we have isolated two new cosmid clones containing the receptor gene. No polymorphic microsatellites were present in the cosmids. However, one cosmid revealed a new TaqI RFLP that showed tight linkage to new highly polymorphic microsatellites for the loci D5S76, D5S39, and D5S6 in seven British and Icelandic reference pedigrees (maximum LOD of 13.2 with D5S76). © 1991.

引用

页码：767 / 769

页数：3

共 14 条

[1] AN ANONYMOUS HUMAN SINGLE COPY GENOMIC CLONE, D5S6 (M4) ON CHROMOSOME-5 IDENTIFIES A 3 ALLELE RFLP [J].

DIETZSCH, E ;

RETIEF, AE ;

LOTZE, MJ ;

WARNICH, L ;

NICHOLSON, DL ;

FOX, MF ;

FRICKE, J ;

DUPLESSIS, L ;

OOSTHUIZEN, CJJ .

NUCLEIC ACIDS RESEARCH, 1986, 14 (04) :1923-1923

[2]

Douglas W. W., 1980, PHARMACOL BASIS THER, P609

[3] THE POLYDEOXYADENYLATE TRACT OF ALU REPETITIVE ELEMENTS IS POLYMORPHIC IN THE HUMAN GENOME [J].

ECONOMOU, EP ;

BERGEN, AW ;

WARREN, AC ;

ANTONARAKIS, SE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (08) :2951-2954

[4] THE GENOMIC CLONE G-21 WHICH RESEMBLES A BETA-ADRENERGIC-RECEPTOR SEQUENCE ENCODES THE 5-HT1A RECEPTOR [J].

FARGIN, A ;

RAYMOND, JR ;

LOHSE, MJ ;

KOBILKA, BK ;

CARON, MG ;

LEFKOWITZ, RJ .

NATURE, 1988, 335 (6188) :358-360

[5] DELETION MAPPING OF DNA MARKERS TO A REGION OF CHROMOSOME-5 THAT COSEGREGATES WITH SCHIZOPHRENIA [J].

GILLIAM, TC ;

FREIMER, NB ;

KAUFMANN, CA ;

POWCHIK, PP ;

BASSETT, AS ;

BENGTSSON, U ;

WASMUTH, JJ .

GENOMICS, 1989, 5 (04) :940-944

[6] REVISION OF CONSENSUS SEQUENCE OF HUMAN ALU REPEATS - A REVIEW [J].

KARIYA, Y ;

KATO, K ;

HAYASHIZAKI, Y ;

HIMENO, S ;

TARUI, S ;

MATSUBARA, K .

GENE, 1987, 53 (01) :1-10

[7] A SAC-1 RFLP IS DETECTED WITH THE 5-HT1A SEROTONIN RECEPTOR PROBE-G21 [J].

KHAN, MAR ;

ISENBERG, KE ;

ASCHAUER, H ;

DEVOR, EJ .

NUCLEIC ACIDS RESEARCH, 1990, 18 (03) :691-691

[8] AN INTRONLESS GENE ENCODING A POTENTIAL MEMBER OF THE FAMILY OF RECEPTORS COUPLED TO GUANINE-NUCLEOTIDE REGULATORY PROTEINS [J].

KOBILKA, BK ;

FRIELLE, T ;

COLLINS, S ;

YANGFENG, T ;

KOBILKA, TS ;

FRANCKE, U ;

LEFKOWITZ, RJ ;

CARON, MG .

NATURE, 1987, 329 (6134) :75-79

[9]

LEYSEN JE, 1988, NEUROMETHODS NEUROPH, V2

[10]

LITT M, 1989, AM J HUM GENET, V44, P397

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