A RAPID HPLC ASSAY FOR ZEARALENONE IN LABORATORY CULTURES OF FUSARIUM-GRAMINEARUM

被引：6

作者：

MERINO, M ^{[1
]}

RAMOS, AJ ^{[1
]}

HERNANDEZ, E ^{[1
]}

机构：

[1] UNIV POLYTECN VALENCIA, POLYTECH, DEPT BIOTECHNOL, E-46010 VALENCIA, SPAIN

来源：

MYCOPATHOLOGIA | 1993年 / 121卷 / 01期

关键词：

CORN; FUSARIUM-GRAMINEARUM; HPLC; ZEARALENONE;

D O I：

10.1007/BF01103351

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A high pressure liquid chromatographic (HPLC) method to determine zearalenone in corn contaminated with Fusarium graminearum is described. After extraction with methanol-water and solvent partition, samples were cleaned up by applying the extract to a disposable silica cartridge and by eluting the toxin with a mixture of hexane/dry ethyl ether (5/5). Separation was achieved by a reverse phase muBondapak C18 column followed by fluorescence detection using an excitation wavelength at 274 nm and an emission wavelength at 440 nm. Detection limit was about 5 ng. Recoveries ranging from 85.37 to 100.97%, in standard solutions range 30-0.5 mug/ml, were found.

引用

页码：27 / 32

页数：6

共 19 条

[1]

BATTALICO A, 1985, APPL ENVIRON MICROB, V49, P547

[2]

BENNETT GA, 1985, J ASSOC OFF ANA CHEM, V68, P958

[3]

CHAMKASEM N, 1989, J ASSOC OFF ANA CHEM, V72, P336

[4]

CHANG K, 1979, AM J VET RES, V40, P1260

[5]

COHEN H, 1980, J ASSOC OFF ANA CHEM, V63, P642

[6]

ENGELHARDT G, 1986, Z LEBENSM UNTERS FOR, V182, P336

[7]

GIMENO A, 1983, J ASSOC OFF ANA CHEM, V66, P565

[8]

HOWELL MV, 1981, J ASSOC OFF ANA CHEM, V64, P1356

[9] CHEMOTAXONOMY OF GIBBERELLA-ZEAE WITH SPECIAL REFERENCE TO PRODUCTION OF TRICHOTHECENES AND ZEARALENONE [J].

ICHINOE, M ;

KURATA, H ;

SUGIURA, Y ;

UENO, Y .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 46 (06) :1364-1369

[10]

MOREAU C, 1979, MOULDS TOXINS FOODS, P234

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