GROWTH OF OLFACTORY EPITHELIAL TISSUE INVITRO - LECTIN STAINING OF AXONS

被引:16
作者
FARBMAN, AI
BUCHHOLZ, JA
机构
[1] Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois
关键词
ORGAN CULTURE; GLYCOCONJUGATE; CONCANAVALIN-A; WHEAT GERM AGGLUTININ; SOYBEAN AGGLUTININ;
D O I
10.1002/jemt.1070230207
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
In vitro methods have been used to study several aspects of development of olfactory epithelium. In this paper, the value of growing olfactory tissue in explant cultures is reviewed and some experiments are reported on the identification of lectin receptors on olfactory axons by the use of lectin-gold complexes. Both concanavalin A-gold (con A-gold) and wheat germ agglutinin-gold consistently decorated olfactory axons in explant cultures. Con A-gold also bound to the tips of growth cone filopodia, suggesting the glycoconjugate molecules containing alpha-methyl-pyranoside are important in adherence of growth cones to their substrate. The wide range in density of lectin-gold particles suggested that axons, and the sensory cells from which they arise, are not a uniform population, i.e., they have different molecular fingerprints. This was supported by the observation that soybean agglutinin-gold stained some axons very well, but others remained unstained. Peanut agglutinin did not bind to any axons.
引用
收藏
页码:173 / 180
页数:8
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