MONITORING AND MODELING DENSITY-DEPENDENT GROWTH OF ANCHORAGE-DEPENDENT CELLS

被引：18

作者：

RUAAN, RC

TSAI, GJ

TSAO, GT

机构：

[1] PURDUE UNIV,SCH CHEM ENGN,W LAFAYETTE,IN 47907

[2] PURDUE UNIV,RENEWABLE RESOURCES ENGN LAB,W LAFAYETTE,IN 47907

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1993年 / 41卷 / 03期

关键词：

DENSITY-DEPENDENT GROWTH; ANCHORAGE-DEPENDENT CELLS; IMAGE ANALYSIS; CHO CELLS; MODEL SIMULATION;

D O I：

10.1002/bit.260410313

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The density-dependent growth of Chinese hamster ovary (CHO) cells was monitored on-line by using an inverted microscope. A flow system was employed for cell cultivation so that nutrient concentration could be maintained and metabolic wastes were removed. With the help of video image analysis, local cell density could be accurately calculated, and cell motility and exposed cell surface area could be estimated. A computer program which accounted for change of cell size and translocation of cells was developed to simulate cell growth. The simulated results of the population dynamics and the variations in cell size showed good agreement with our experimental observations. Cell motility and initial cell distribution on the substratum were found to have strong effects on cell growth.

引用

页码：380 / 389

页数：10

共 11 条

[1]

Alberts B, 2017, MOL BIOL CELL

[2] MODELING OF CONTACT-INHIBITED ANIMAL-CELL GROWTH ON FLAT SURFACES AND SPHERES [J].

CHERRY, RS ;

PAPOUTSAKIS, ET .

BIOTECHNOLOGY AND BIOENGINEERING, 1989, 33 (03) :300-305

[3] REQUIREMENT OF SERUM FOR DNA SYNTHESIS IN BHK 21 CELLS - EFFECTS OF DENSITY, SUSPENSION AND VIRUS TRANSFORMATION [J].

CLARKE, GD ;

STOKER, MGP ;

LUDLOW, A ;

THORNTON, M .

NATURE, 1970, 227 (5260) :798-&

[4] TOPOINHIBITION AND SERUM REQUIREMENT OF TRANSFORMED AND UNTRANSFORMED CELLS [J].

DULBECCO, R .

NATURE, 1970, 227 (5260) :802-&

[5] A MODEL FOR DENSITY-DEPENDENT GROWTH OF ANCHORAGE-DEPENDENT MAMMALIAN-CELLS [J].

FRAME, KK ;

HU, WS .

BIOTECHNOLOGY AND BIOENGINEERING, 1988, 32 (08) :1061-1066

[6] CONTACT INHIBITION OF CELL DIVISION IN 3T3 CELLS [J].

HOLLEY, RW ;

KIERNAN, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1968, 60 (01) :300-&

[7]

Lackie J.M., 1986, CELL MOVEMENT CELL B, P253, DOI [10.1007/978-94-009-4071-0_10, DOI 10.1007/978-94-009-4071-0_10]

[8] THE INFLUENCE OF SHEAR-STRESS ON CULTURED VASCULAR ENDOTHELIAL-CELLS - THE STRESS RESPONSE OF AN ANCHORAGE-DEPENDENT MAMMALIAN-CELL [J].

LEVESQUE, MJ ;

SPRAGUE, EA ;

SCHWARTZ, CJ ;

NEREM, RM .

BIOTECHNOLOGY PROGRESS, 1989, 5 (01) :1-8

[9] A STOCHASTIC-MODEL TO SIMULATE THE GROWTH OF ANCHORAGE DEPENDENT CELLS ON FLAT SURFACES [J].

LIM, JHF ;

DAVIES, GA .

BIOTECHNOLOGY AND BIOENGINEERING, 1990, 36 (06) :547-562

[10]

RUAAN RC, 1991, THESIS PURDUE U W LA

← 1 2 →