CARBOXYLATED LATEXES FOR COVALENT COUPLING ANTIBODIES .1.

被引:58
作者
BASTOSGONZALEZ, D [1 ]
ORTEGAVINUESA, JL [1 ]
NIEVES, FJD [1 ]
HIDALGO-ALVAREZ, R [1 ]
机构
[1] UNIV GRANADA, DEPT APPL PHYS, BIOCOLLOID & FLUID PHYS GRP, E-18071 GRANADA, SPAIN
关键词
POLYMER COLLOIDS; ELECTROKINETICS;
D O I
10.1006/jcis.1995.0026
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The aim of the present work is to prepare and characterize carboxylated latexes (CLs) in order to bind antibodies covalently and efficiently. Carboxylated latexes were prepared by surfactant-free emulsion polymerization using an ate-initiator, which provides carboxyl end groups on the latex surface directly. Two latexes were characterized using different techniques: particle size by electron microscopy and photocorrelation spectroscopy, surface charge density by conductimetric and potentiometric titrations, and electrophoretic mobility versus pH and ionic strength (KBr). The colloidal stability of both latexes shows that these carboxylated latexes can be used for biomedical applications. Activation of carboxyl end groups was performed using the carbodiimide method, and the best conditions for covalent coupling of IgG were obtained. (C) 1995 Academic Press, Inc.
引用
收藏
页码:232 / 239
页数:8
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