CYTOLOGY OF CALLUS OF CRYPTOMERIA-JAPONICA

被引：12

作者：

MEHRA, PN ^{[1
]}

ANAND, M ^{[1
]}

机构：

[1] PANJAB UNIV, DEPT BOT, CHANDIGARH 160014, INDIA

来源：

PHYSIOLOGIA PLANTARUM | 1979年 / 45卷 / 01期

关键词：

D O I：

10.1111/j.1399-3054.1979.tb01676.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Callusing has been initiated from the cotyledons of 4‐week‐old seedlings of Cryptomeria japonica on Murashige and Skoog's medium supplemented with 22.8 μM IAA. Callus was grown successfully for more than 2 years on the same medium without any decline in growth vigour. The callus has shown differentiation of tracheids with reticulate thickenings but differentiation of root, shoot or whole plant has not yet been observed. Cytology of 2‐month‐old callus showed the presence of cells containing normal diploid chromosome complement of 2n = 22 and normal mitosis. However, the 6‐month‐old callus revealed majority of cells with normal diploid complement and the usual karyotype, but a few cells were noticed with aneuploid numbers ranging from 2n = 15–17. Anaphase bridges and lagging chromosomes have also been encountered. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：127 / 131

页数：5

共 19 条

[1] CHROMOSOME NUMBERS IN SINGLE CELL CLONES OF TOBACCO TISSUE [J].