BRADYKININ SYNERGISTICALLY POTENTIATES INTERLEUKIN-1 INDUCED BONE-RESORPTION AND PROSTANOID BIOSYNTHESIS IN NEONATAL MOUSE CALVARIAL BONES

Interleukin-1 (IL-1) α and β dose-dependently stimulated the release of 45Ca and the formation of prostaglandin E2 (PGE2) and PGI2 in cultured mouse calvarial bones, with IL-1 β being the most potent agonist. Bradykinin (BK; 10 nmol/l) synergistically potentiated the effect of IL-1 α (10 pg/ml) and IL-1 β (5 pg/ml) both on 45Ca release and on biosynthesis of PGE2 and PGI2. The capacity of BK to potentiate IL-1 β induced 45Ca release and PGE2 formation was seen at concentrations of BK from 1-1000 nmol/l. These data indicate that BK and IL-1, which are formed in inflammatory processes, may act in concert to stimulate bone resorption in the vicinity of inflammatory lesions. © 1991.