DYNAMIC ASSOCIATION BETWEEN ARTERY SHEAR-FLOW CONDITION AND PLATELET CYTOSOLIC FREE CA2+CONCENTRATION IN HUMAN HYPERTENSION

被引:22
作者
LEVENSON, J
DEVYNCK, MA
PITHOISMERLI, I
SANG, KHL
FILITTI, V
SIMON, A
机构
[1] Centre de Diagnostic, Hopital Broussais, 75674 Paris 14, 96, Rue Didot
关键词
D O I
10.1042/cs0790613
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Blood cells and vascular endothelial cells are subjected to a wide range of haemodynamically generated shear stress forces. In vitro, membrane stretching or shear stress have been observed to activate ion channels and cell metabolism and to facilitate erythrocyte and platelet aggregation. The present study was designed to evaluate the participation of shear stresses in the control of apparent platelet cytosolic free Ca2+ concentration in hypertensive patients. Shear conditions and platelet cytosolic free Ca2+ concentration in vitro were studied after a dynamic perturbation induced by 3 months of double-blind treatment with one of two β-antagonists, carteolol and atenolol. Brachial artery wall shear rate and stress were estimated by means of a pulsed Doppler velocimeter, and blood viscosity was measured by a co-axial viscometer at a shear rate of 95 s-1. Platelet cytosolic free Ca2+ concentration was simultaneously measured by using the Quin-2 fluorescent chelator. The direct effect of atenolol and carteolol on platelet cytosolic free Ca2+ concentration in vitro was also measured after addition of the β-blockers to platelet-rich plasma. Atenolol and carteolol decreased blood pressure similarly but their effects on shear rate (P < 0.02), shear stress (P < 0.01) and platelet cytosolic free Ca2+ concentration (P < 0.05) differed after 3 months of therapy. In contrast, neither of the drugs significantly altered platelet cytosolic free Ca2+ concentration, in vitro per se. In the overall population, strong positive correlations existed not only between changes in platelet cytosolic free Ca2+ concentration and those in shear rate (r = 0.81, P < 0.001) and shear stress (r = 0.83, P < 0.001), but also between their absolute values, suggesting a possible haemodynamic shear-dependent modulation of transmembrane Ca2+ transport.
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页码:613 / 618
页数:6
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