APPLICATION OF POLYSTYRENE-BOUND INVERTASE TO CONTINUOUS SUCROSE HYDROLYSIS ON PILOT SCALE

被引:18
作者
MANSFELD, J
SCHELLENBERGER, A
ROMBACH, J
机构
[1] UNIV HALLE SAALE,INST BIOCHEM,DEPT ENZYMOL,WEINBERGWEG 16A,O-4050 HALLE,GERMANY
[2] TECH UNIV KOTHEN,DEPT BIOL,KOTHEN,GERMANY
关键词
INVERTASE; SUCROSE HYDROLYSIS; POLYSTYRENE RESIN; IMMOBILIZATION;
D O I
10.1002/bit.260400902
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Invertase from baker's yeast (Saccharomyces cerevisiae) covalently bound to a macroporous polystyrene anion-exchange resin via glutaraldehyde was applied to continuous sucrose hydrolysis in packed-bed reactors. The process was scaled up from 3-mL laboratory reactors via 0.3-L reactors to pilot-scale 50-L reactors without significant loss of efficiency. The described process allows the production of a wide spectrum of invert sugar syrups with high purity in a continuous procedure. The 50-L reactor was used under process conditions 1 year without significant loss of productivity at a temperature of 40-degrees-C. A productivity of 760 g/h was obtained with 1 L invertase-polystyrene complex using a 2.5M sucrose solution as substrate.
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页码:997 / 1003
页数:7
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