CLONING AND CHARACTERIZATION OF A NOVEL BACILLUS-THURINGIENSIS CYTOLYTIC DELTA-ENDOTOXIN

被引：57

作者：

KONI, PA ^{[1
]}

ELLAR, DJ ^{[1
]}

机构：

[1] UNIV CAMBRIDGE, DEPT BIOCHEM, TENNIS COURT RD, CAMBRIDGE CB2 1QW, ENGLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 229卷 / 02期

基金：

英国惠康基金;

关键词：

BACILLUS-THURINGIENSIS; DELTA-ENDOTOXIN; CLONING; SEQUENCE; EXPRESSION;

D O I：

10.1006/jmbi.1993.1037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene encoding a major entomocidal polypeptide from Bacillus thuringiensis subspecies kyushuensis Δ-endotoxin crystals (CytB) was cloned into Escherichia coli and sequenced. The deduced amino acid sequence gave a predicted molecular mass of 29 236 Da and showed 39% identity and 70% similarity with the 27,371 Da CytA protein from Bacillus thuringiensis subspecies israelensis. The larger size of CytB compared to CytA appears to be due to additional sequence in CytB after the CytA C-terminus. Unlike CytA, CytB was freely expressed in Escherichia coli and formed cytoplasmic inclusions without the need for a “helper” protein. Electron microscopic observation of CytB inclusions revealed them to be generally amorphous, but examples possessing some lattice structure were seen. © 1993 Academic Press, Inc.

引用

页码：319 / 327

页数：9

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