MOLECULAR-CLONING OF AN ESSENTIAL YEAST GENE ENCODING A PROTEASOMAL SUBUNIT

被引：21

作者：

GEORGATSOU, E

GEORGAKOPOULOS, T

THIREOS, G

机构：

[1] Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology, Heraclio, 711 10 Crete

来源：

FEBS LETTERS | 1992年 / 299卷 / 01期

关键词：

PUP2; PROTEASOME; YEAST; SACCHAROMYCES-CEREVISIAE;

D O I：

10.1016/0014-5793(92)80095-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present the cloning and sequence of a Saccharomyces cerevisiae gene, PUP2, which encodes for a proteasomal subunit. The PUP2 protein is similar to other proteasomal components from yeast, as well as from Drosophila and rat. Although not-properly-folded proteins have been implicated to constitute substrates of proteasomes, we show that the accumulation of such proteins does not induce expression of the PUP2 gene. Finally, gene disruption experiments demonstrate that PUP2 belongs to the class of yeast proteasomal subunits that are essential for cell viability.

引用

页码：39 / 43

页数：5

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