IDENTIFICATION OF THE REACTION-PRODUCTS OF ROSMARINIC ACID SYNTHASE FROM CELL-CULTURES OF COLEUS-BLUMEI BY ION SPRAY MASS-SPECTROMETRY AND TANDEM MASS-SPECTROMETRY

被引：9

作者：

PETERSEN, M

METZGER, JW

机构：

[1] Institut Für Entwicklungs- Und Molekularbiologie Der Pflanzen, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, D-4000

[2] Institut Für Organische Chemie, Universität Tübingen, Tübingen, D-7400

来源：

PHYTOCHEMICAL ANALYSIS | 1993年 / 4卷 / 03期

关键词：

COLEUS-BLUMEI; HYDROXYCINNAMIC ACID ESTERS; ION SPRAY MASS SPECTROMETRY; PLANT SECONDARY METABOLISM; ROSMARINIC ACID SYNTHASE; TANDEM MASS SPECTROMETRY;

D O I：

10.1002/pca.2800040310

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Different substrates were used in reaction assays for rosmarinic acid synthase, an enzyme involved in rosmarinic acid biosynthesis in cell cultures of Coleus blumei. In order to introduce the cinnamoyl moiety of the ester, 4-coumaroyl-CoA and caffeoyl-CoA were applied as substrates; for the hydroxyphenyllactic acid moiety, 4-hydroxyphenyllactate and 3,4-dihydroxyphenyllactate were used. Four different reaction products were thus produced and these were analysed by ion spray mass spectrometry and tandem mass spectrometry after purification by semi-preparative high pressure liquid chromatography. The molecular masses and the fragmentation patterns obtained by tandem mass spectrometry of the reaction products formed by the catalysis of rosmarinic acid synthase were in agreement with the structures of rosmarinic acid (caffeoyl-3',4'-dihydroxyphenyllactate) and rosmarinic acid-like esters (4-coumaroyl-4'-hydroxyphenyllactate, 4-coumaroyl-3',4'-dihydroxyphenyllactate, caffeoyl-4'-hydroxyphenyllactate) as expected for the substrates used in the enzyme reaction.

引用

页码：131 / 134

页数：4

共 9 条

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