EFFECT OF ALCOHOL ON THE FUNCTIONS OF KUPFFER CELLS AND SPLENIC MACROPHAGES IN RATS

被引：16

作者：

FUKUI, H ^{[1
]}

KITANO, H ^{[1
]}

TSUJII, T ^{[1
]}

MORIMURA, M ^{[1
]}

KIKUCHI, E ^{[1
]}

MATSUMOTO, M ^{[1
]}

TSUJITA, S ^{[1
]}

KIKUKAWA, M ^{[1
]}

NAGAMOTO, I ^{[1
]}

NAKATANI, T ^{[1
]}

OKAMOTO, Y ^{[1
]}

机构：

[1] NARA MED UNIV,DEPT CLINICOLAB DIAGNOST,KASHIHARA,NARA 634,JAPAN

来源：

ALCOHOL AND ALCOHOLISM | 1993年 / 28卷

关键词：

D O I：

10.1093/alcalc/28.Supplement_1B.53

中图分类号：

R194 [卫生标准、卫生检查、医药管理];

学科分类号：

摘要：

Uptake of endotoxin and production of tumor necrosis factor (TNF) by Kupffer cells and splenic macrophages were measured in chronically alcohol-fed rats. Twenty male Sprague-Dawley rats, weighing 200-250 g, were pair-fed by isocaloric control and ethanol-containing Lieber-DeCarli liquid diets for 6 weeks. Endotoxin uptake and TNF production of Kupffer cells and splenic macrophages in the chronic ethanol load-group were significantly (P < 0.001) greater than those in the control group. The increase in endotoxin uptake was more prominent in Kupffer cells and the increase in TNF production was more marked in splenic macrophages int he chronic ethanol group. When these cells were precultured in the medium containing 10-100 mM ethanol, the endotoxin uptake and TNF production of Kupffer cells isolated from control and chronic ethanol-fed rats were decreased in proportion to the concentration of ethanol in the culture medium. However, the addition of ethanol to the culture medium did not affect the endotoxin uptake and TNF production of splenic macrophages. These results support the hypothesis that the splenic macrophages are important for endotoxin uptake, and excessive production of TNF in rats given large amounts of alcohol.

引用

页码：53 / 57

页数：5

共 23 条

[1] ALI MV, 1967, J LAB CLIN MED, V70, P295
[2] ENDOTOXIN-INDUCED HYPERCOAGULABILITY - A POSSIBLE AGGRAVATING FACTOR OF ALCOHOLIC LIVER-DISEASE
ARAI, M
NAKANO, S
OKUNO, F
HIRANO, Y
SUJITA, K
KOBAYASHI, T
ISHII, H
TSUCHIYA, M
[J]. HEPATOLOGY, 1989, 9 (06) : 846 - 851
[3] BJARNASON I, 1984, LANCET, V1, P179
[4] ENDOTOXEMIA IN PATIENTS WITH ALCOHOLIC AND NONALCOHOLIC CIRRHOSIS AND IN SUBJECTS WITH NO EVIDENCE OF CHRONIC LIVER-DISEASE FOLLOWING ACUTE ALCOHOL EXCESS
BODE, C
KUGLER, V
BODE, JC
[J]. JOURNAL OF HEPATOLOGY, 1987, 4 (01) : 8 - 14
[5] ENDOTOXIN-INDUCED SERUM FACTOR THAT CAUSES NECROSIS OF TUMORS
CARSWELL, EA
OLD, LJ
KASSEL, RL
GREEN, S
FIORE, N
WILLIAMSON, B
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (09) : 3666 - 3670
[6] PLASMA ENDOTOXIN CONCENTRATIONS IN PATIENTS WITH ALCOHOLIC AND NONALCOHOLIC LIVER-DISEASE - REEVALUATION WITH AN IMPROVED CHROMOGENIC ASSAY
FUKUI, H
BRAUNER, B
BODE, JC
BODE, C
[J]. JOURNAL OF HEPATOLOGY, 1991, 12 (02) : 162 - 169
[7] FUKUI H, 1993, ALCOHOL ALCOHOLISM S, P65
[8] HONCHEL R, 1991, J LAB CLIN MED, V117, P202
[9] KHORUTS A, 1991, HEPATOLOGY, V13, P267, DOI 10.1002/hep.1840130211
[10] LE JM, 1987, LAB INVEST, V56, P234

← 1 2 3 →