ANTIMICROBIAL ACTIVITY OF WATER-SOLUBLE PRODUCTS DERIVED FROM RADISH MUSTARD OIL AND IDENTIFICATION OF AN ACTIVE COMPONENT THEREIN

Antimicrobial property of water-soluble products obtained by degradation of 4-methyl-thio-3-butenyl isothiocyanate, the pungent principle of radish, was studied using 5 Gramnegative and 5 Gram-positive bacteria, 5 yeasts, and 9 fungi. Two antimicrobial components were found in the water-soluble products, of which the major one was isolated and indentified to be 2-thioxo-3-pyrrolidinecarbaldehyde on the bases of UV, MS, H-1-NMR and C-13-NMR spectral data. The identified compound exhibited a prominent growth-inhibition on the fungi and Gram-positive bacteria. The inhibitory effect on the yeasts and Gramnegative bacteria except for Escherichia coli was the least among the tested microorganisms.