A WESTERN-BLOT TO DETECT ANTIBODY TO AVIAN REOVIRUS

被引：12

作者：

ENDOMUNOZ, LB ^{[1
]}

机构：

[1] NATL BIOL STAND LAB,PARKVILLE,VIC 3052,AUSTRALIA

来源：

AVIAN PATHOLOGY | 1990年 / 19卷 / 03期

关键词：

D O I：

10.1080/03079459008418701

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Purified reoviruses RAM-1 and 74/2 were combined and used as antigen. The Western blot detected antibodies to 16/16 avian reoviruses in sera from experimentally inoculated chickens, and to 6/7 reoviruses in contact sera from naturally infected chickens. The test was specific for avian reovirus and was more sensitive and specific than immunofluorescence. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

收藏

页码：477 / &

相关论文

共 9 条

[1] SEROLOGICAL STUDIES WITH REOVIRUSES IN CHICKENS, TURKEYS AND DUCKS [J].

ADAIR, BM ;

BURNS, K ;

MCKILLOP, ER .

JOURNAL OF COMPARATIVE PATHOLOGY, 1987, 97 (05) :495-501

[2]

BAGUST TJ, 1975, AUST VET J, V51, P406

[3] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[4] ISOLATION OF AN AVIAN REOVIRUS [J].

MUSTAFFABABJEE, A ;

SPRADBROW, PB .

AUSTRALIAN VETERINARY JOURNAL, 1971, 47 (06) :284-+

[5] AVIAN REOVIRUS POLYPEPTIDES - ANALYSIS OF INTRACELLULAR VIRUS-SPECIFIED PRODUCTS, VIRIONS, TOP COMPONENT, AND CORES [J].

SCHNITZER, TJ ;

RAMOS, T ;

GOUVEA, V .

JOURNAL OF VIROLOGY, 1982, 43 (03) :1006-1014

[6] ADAPTATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY TO THE AVIAN SYSTEM [J].

SLAGHT, SS ;

YANG, TJT ;

VANDERHEIDE, L .

JOURNAL OF CLINICAL MICROBIOLOGY, 1979, 10 (05) :698-702

[7] POLYPEPTIDE COMPONENTS OF VIRIONS, TOP COMPONENT AND CORES OF REOVIRUS TYPE-3 [J].

SMITH, RE ;

ZWEERINK, HJ ;

JOKLIK, WK .

VIROLOGY, 1969, 39 (04) :791-&

[8] PHYSICAL AND CHEMICAL CHARACTERIZATION OF AN AVIAN REOVIRUS [J].

SPANDIDOS, DA ;

GRAHAM, AF .

JOURNAL OF VIROLOGY, 1976, 19 (03) :968-976

[9]

[No title captured]