USE OF IMMUNOADSORBENTS FOR THE STUDY OF ANTIBODY-BINDING TO SPERM WHALE MYOGLOBIN AND ITS SYNTHETIC ANTIGENIC SITES

被引:81
作者
TWINING, SS [1 ]
ATASSI, MZ [1 ]
机构
[1] MAYO MED SCH,DEPT IMMUNOL,ROCHESTER,MN 55901
关键词
D O I
10.1016/0022-1759(79)90088-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Conditions for preparing immunoadsorbents of sperm-whale myoglobin and its five synthetic antigenic sites and for desorption of radiolabeled antibodies from the immunoadsorbents were studied. In immunoadsorbent titration studies, the sum of the amounts of antibodies bound in the plateau (maximum binding) by the adsorbents of the five sites accounted quantitatively for the entire (100%) antibody response to sperm-whale myoglobin. © 1979.
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页码:139 / 151
页数:13
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