A MEDIUM DETECTING BETA-GLUCURONIDASE FOR THE SIMULTANEOUS MEMBRANE FILTRATION ENUMERATION OF ESCHERICHIA-COLI AND COLIFORMS FROM DRINKING-WATER

被引：46

作者：

SARTORY, DP

HOWARD, L

机构：

[1] Severn Trent Water Ltd, Shrewsbury, SY3 8BJ, Shelton

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1992年 / 15卷 / 06期

关键词：

D O I：

10.1111/j.1472-765X.1992.tb00782.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A medium for the single membrane enumeration of Escherichia coli and coliforms from potable water was developed by the modification of the standard UK membrane filtration medium. The medium, membrane-Lactose Glucuronide Agar (m-LGA), employs a chromogenic substrate for the detection of beta-glucuronidase activity and sodium pyruvate to enhance recovery of chlorine-stressed coliforms. Escherichia coli identification was significantly improved on m-LGA with 98.6% of presumptive isolates confirming. Recovery of coliforms from drinking water samples was also significantly improved.

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页码：273 / 276

页数：4

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