ACQUISITION OF APPARENT DNA SLIPPAGE STRUCTURES DURING EXTENSIVE EVOLUTIONARY DIVERGENCE OF PCAD AND CATD GENES ENCODING IDENTICAL CATALYTIC ACTIVITIES IN ACINETOBACTER-CALCOACETICUS

被引:25
作者
HARTNETT, GB [1 ]
ORNSTON, LN [1 ]
机构
[1] YALE UNIV, DEPT BIOL, NEW HAVEN, CT 06511 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
EVOLUTION; MUTATION; G+C CONTENT; SELECTION; RECOMBINATION; ALPHA/BETA-FOLD HYDROLASES; BETA-KETOADIPATE ENOL-LACTONE HYDROLASE;
D O I
10.1016/0378-1119(94)90350-6
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The pea operon from the Gram(-) bacterium Acinetobacter calcoaceticus encodes all of the enzymes required for catabolism of protocatechuate to common intermediary metabolites. This report presents the 2754-nucleotide (nt) sequence of a HindIII restriction fragment containing pcaD, the 801-bp gene encoding beta-ketoadipate enol-lactone hydrolase I. The deduced primary structure of A. calcoaceticus PcaD shares 44% amino acid (aa) sequence identity with the aligned primary structure of CatD (beta-ketoadipate enol-lactone hydrolase II) from the same organism, and the overall nt sequence identity of the two genes is 51.8%. In the 56% of the genes where selection for identical aa residues was not imposed, pcaD and catD have diverged so extensively that nt sequence identity of the aligned segments is only 28.2%; the G+C contents of these segments from the respective genes differ by 8%. Conserved within the aligned PcaD and CatD aa sequences is a Ser residue corresponding to the nucleophile within the alpha/beta-fold of many hydrolytic enzymes. In this region of primary structure, PcaD and CatD appear to have maintained some different aa sequences derived from a common ancestor. Conservation of the different aa sequences during extreme evolutionary divergence suggests that separate segments of primary structure, conserved within either PcaD or CatD, may be functionally incompatible within recombinant enzymes. Consequently, selection for avoidance of genetic exchange between pcaD and catD could account for the thorough nt substitution in regions where identical aa were not selected. Sequence repetitions within pcaD suggest that the multiple mutations required for its extensive divergence from catD were achieved in part by acquisition of a complex DNA slippage structure.
引用
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页码:23 / 29
页数:7
相关论文
共 23 条
[1]   EVOLUTIONARY DIVERGENCE OF POBA, THE STRUCTURAL GENE ENCODING P-HYDROXYBENZOATE HYDROXYLASE IN AN ACINETOBACTER-CALCOACETICUS STRAIN WELL-SUITED FOR GENETIC-ANALYSIS [J].
DIMARCO, AA ;
AVERHOFF, BA ;
KIM, EE ;
ORNSTON, LN .
GENE, 1993, 125 (01) :25-33
[2]   INFLUENCE OF THE CATBCE SEQUENCE ON THE PHENOTYPIC REVERSION OF A PCAE MUTATION IN ACINETOBACTER-CALCOACETICUS [J].
DOTEN, RC ;
GREGG, LA ;
ORNSTON, LN .
JOURNAL OF BACTERIOLOGY, 1987, 169 (07) :3175-3180
[3]   CLONING AND GENETIC ORGANIZATION OF THE PCA GENE-CLUSTER FROM ACINETOBACTER-CALCOACETICUS [J].
DOTEN, RC ;
NGAI, KL ;
MITCHELL, DJ ;
ORNSTON, LN .
JOURNAL OF BACTERIOLOGY, 1987, 169 (07) :3168-3174
[4]  
GREGGJOLLY LA, 1991, THESIS YALE U NEW HA
[5]   POTENTIAL DNA SLIPPAGE STRUCTURES ACQUIRED DURING EVOLUTIONARY DIVERGENCE OF ACINETOBACTER-CALCOACETICUS CHROMOSOMAL BENABC AND PSEUDOMONAS-PUTIDA TOL PWWO PLASMID XYLXYZ, GENES ENCODING BENZOATE DIOXYGENASES [J].
HARAYAMA, S ;
REKIK, M ;
BAIROCH, A ;
NEIDLE, EL ;
ORNSTON, LN .
JOURNAL OF BACTERIOLOGY, 1991, 173 (23) :7540-7548
[6]   DNA-SEQUENCES OF GENES ENCODING ACINETOBACTER-CALCOACETICUS PROTOCATECHUATE 3,4-DIOXYGENASE - EVIDENCE INDICATING SHUFFLING OF GENES AND OF DNA-SEQUENCES WITHIN GENES DURING THEIR EVOLUTIONARY DIVERGENCE [J].
HARTNETT, C ;
NEIDLE, EL ;
NGAI, KL ;
ORNSTON, LN .
JOURNAL OF BACTERIOLOGY, 1990, 172 (02) :956-966
[7]  
HARTNETT GB, 1992, THESIS YALE U NEW HA
[8]   CODON USAGE AND TRANSFER-RNA CONTENTS - ORGANISM-SPECIFIC CODON-CHOICE PATTERNS IN REFERENCE TO THE ISOACCEPTOR CONTENTS [J].
IKEMURA, T ;
OZEKI, H .
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1982, 47 :1087-1097
[9]  
KAPLAN JB, 1984, MOL BIOL EVOL, V1, P456
[10]   CLONING AND SEQUENCING OF 2 TANDEM GENES INVOLVED IN DEGRADATION OF 2,3-DIHYDROXYBIPHENYL TO BENZOIC-ACID IN THE POLYCHLORINATED BIPHENYL-DEGRADING SOIL BACTERIUM PSEUDOMONAS SP STRAIN KKS102 [J].
KIMBARA, K ;
HASHIMOTO, T ;
FUKUDA, M ;
KOANA, T ;
TAKAGI, M ;
OISHI, M ;
YANO, K .
JOURNAL OF BACTERIOLOGY, 1989, 171 (05) :2740-2747