DETECTION OF GUANYL CYCLASE IN MAMMALIAN TISSUES

被引：203

作者：

WHITE, AA

AURBACH, GD

机构：

[1] Section on Mineral Metabolism, Metabolic Diseases Branch, National Institute of Arthritis and Metabolic Diseases, Bethesda

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 191卷 / 03期

关键词：

D O I：

10.1016/0005-2744(69)90362-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Guanyl cyclase was assayed by determining the rate of conversion of [α-32P]GTP to cyclic 3′,5′-[32P]GMP. A two-step chromatographic procedure was developed for isolating the product. The enzyme was found in the soluble fraction after homogenizing tissues in 0.3 M sucrose. The reaction showed a pH optimum near 7.6 and was dependent on Mn2+. The relative enzymatic activity for tissues of the rat was lung >> brain = spleen > kidney = liver = heart. © 1969.

引用

页码：686 / &

共 23 条

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