REACTION OF 5-S-RNA WITH A RADIOACTIVE CARBODIIMIDE

被引：25

作者：

LEE, JC

INGRAM, VM

机构：

[1] Department of Biology Massachusetts Institute of Technology Cambridge, MA

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1969年 / 41卷 / 03期

关键词：

D O I：

10.1016/0022-2836(69)90286-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Escherichia coli 5 s RNA was reacted with 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl) carbodiimide [14C]methoiodide in the presence of magnesium ion. The RNA molecule seems highly compact in that most of its bases are inaccessible to the carbodiimide. However, the uridine residues in two sequences, UpApGp and ApCpCpCpCpApUpGp, are more exposed and available to reaction with the reagent, which throws light on the conformation of the RNA molecule. © 1969.

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页码：431 / &

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