COVALENTLY LINKED PEPTIDES FOR ENZYME-LINKED-IMMUNOSORBENT-ASSAY

被引：43

作者：

SONDERGARDANDERSEN, J

LAURITZEN, E

LIND, K

HOLM, A

机构：

[1] STATENS SERUM INST,MED BIOTECHNOL RES CTR,DK-2300 COPENHAGEN,DENMARK

[2] STATENS SERUM INST,DEPT RUBELLA,HIV LAB,DK-2300 COPENHAGEN,DENMARK

[3] UNIV COPENHAGEN,HC ORSTED INST,CHEM LAB 2,DK-2100 COPENHAGEN,DENMARK

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 131卷 / 01期

关键词：

Angiotensin II; CovaLink; ELISA; HIV-2; Immobilization; Synthetic peptide;

D O I：

10.1016/0022-1759(90)90238-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A general method is described, by which synthetic peptides are covalently linked via their carboxyl group to microtiter plates (CovaLink) for enzyme-linked immunosorbent assay (ELISA). Plates were prepared by this method with an angiotensin II peptide and with an HIV-2 peptide and attachment detected by rabbit anti-angiotensin serum and with a positive serum from an HIV-2-infected patient, respectively, using the common ELISA procedure in the last steps. The method is simple to perform, it constitutes an alternative to the common ELISA method, and eliminates the risk of inadvertent loss of peptide during the procedure. The method is highly reproducible and has a high sensitivity. It may be used for either antigen or antibody detection. © 1990.

引用

页码：99 / 104

页数：6

共 17 条

[1]

ARNON R, 1987, SYNTHETIC VACCINES, V2

[2]

ARNON R, 1987, SYNTHETIC VACCINES, V1

[3] SYNTHETIC PEPTIDES AS ANTIGENS - PITFALLS OF CONJUGATION METHODS [J].