AUTONOMOUS DEVELOPMENTAL CONTROL OF HUMAN EMBRYONIC GLOBIN GENE SWITCHING IN TRANSGENIC MICE

被引：116

作者：

RAICH, N ^{[1
]}

ENVER, T ^{[1
]}

NAKAMOTO, B ^{[1
]}

JOSEPHSON, B ^{[1
]}

PAPAYANNOPOULOU, T ^{[1
]}

STAMATOYANNOPOULOS, G ^{[1
]}

机构：

[1] UNIV WASHINGTON, DEPT MED GENET, SEATTLE, WA 98195 USA

来源：

SCIENCE | 1990年 / 250卷 / 4984期

关键词：

D O I：

10.1126/science.2251502

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The mechanisms by which expression of the β-like globin genes are developmentally regulated are under intense investigation. The temporal control of human embryonic (ε) globin expression was analyzed. A 3.7-kilobase (kb) fragment that contained the entire human ε-globin gene was linked to a 2.5-kb cassette of the locus control region (LCR), and the developmental time of expression of this construct was studied in transgenic mice. The human e-globin transgene was expressed in yolk sac-derived primitive erythroid cells, but not in fetal liver or bone marrow-derived definitive erytiroid cells. The absence of ε gene expression in definitive erythroid cells suggests that the developmental regulation of the ε-globin gene depends only on the presence of the LCR and the ε-globin gene itself (that is, an autonomous negative control mechanism). The autonomy of ε-globin gene developmental control disfinguishes it from the competitive mechanism of regulation of γ and β-globin genes, and therefore, suggests that at least two distinct mechanisms function in human hemoglobin switching.

引用

页码：1147 / 1149

页数：3

共 21 条

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