ISOLATION OF 2 GENES THAT ENCODE SUBUNITS OF THE YEAST TRANSCRIPTION FACTOR-IIA

被引：121

作者：

RANISH, JA ^{[1
]}

LANE, WS ^{[1
]}

HAHN, S ^{[1
]}

机构：

[1] HARVARD UNIV,HARVARD MICROCHEM FACIL,CAMBRIDGE,MA 02138

来源：

SCIENCE | 1992年 / 255卷 / 5048期

关键词：

D O I：

10.1126/science.1546313

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The yeast transcription factor IIA (TFIIA), a component of the basal transcription machinery of RNA polymerase II and implicated in vitro in regulation of basal transcription, is composed of two subunits of 32 and 13.5 kilodaltons. The genes that encode these subunits, termed TOA1 and TOA2, respectively, were cloned. Neither gene shares obvious sequence similarity with the other or with any other previously identified genes. The recombinant factor bound to a TATA binding protein-DNA complex and complemented yeast and mammalian in vitro transcription systems depleted of TFIIA. Both the TOA1 and TOA2 genes are essential for growth of yeast.

引用

页码：1127 / 1129

页数：3

共 27 条

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